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Various Using of Spectrophotometer on Different Fields of Science


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A spectrophotometer can be a scientific instrument commonly used in research and industrial laboratories. The instrument is frequently employed in the fields of molecular biology, chemistry and biochemistry as it highly describes output of Ultraviolet-Visible Rays. This light energy depends on wavelengths which can be called lambda which may extend more than a specific range. A typical eye would usually reply to wavelengths which extend 390 to 750 nanometers.

The spectrum regarding is similar to the colors of rainbow which in turn defines colors which might be readily interpreted from the brain. A maximum sensitivity for the naked eye is about 555 nm that is across the green area of the optical spectrum. There are also colors who are not readily comprehended through the brain unless blended with multiple wavelengths. Unsaturated colors including pink, magenta or purple are good types of this.

The visible window overlaps the human visible response spectrum. Even though the near infrared (NIR) spectrum is just beyond your human response window as well as the medium and long wavelength are way after dark human region's response. Other species likewise have the same function for visualizing colors like humans. Like the bees as well as other insects, they are able to see ultraviolet rays which help them find nectars in flowers.

Hence, spectrophotometer is essential as a way to look into the spectrum of light that objects emit and absorb. Example, when manufacturing computer monitors; NIR companies would blend red, green and blue within their respective color triangles. But blending of colors isn't easy advertising seems simply because they have various wavelength i.e. blue is 450-474 nm.

Where are Spectrophotometer used?

The samples for Ultra violet rays are often measured in containers called cuvettes. This is often utilized if found in the visible spectrum or for ultraviolet ray measurements. NIR spectrophotometer can often be employed for industrial coloring particularly in textile and ink manufacturers. Moreover, they're also used in for color-sensitive materials used mainly for forensics including printers.

For biological and chemical research, solutions may be measured from the a higher level light absorption with a specific wavelength. Example, molecular biology use NIR spectrometer to determine the energy DNA samples for its compound mixture. This band are brilliant when enzymatic or chemical reactions cause to develop materials to switch overtime. Example of that is oxidation among citrus fruits. A spectrophotometer is advantageous in identifying to what extent oxidation accounts for the brownish effect on fruits.

Lastly, NIR reflectance is employed to ensure the spectroscopic method utilize these materials. Moreover, reflectance is used to signal stretching or bending of chemical molecules like, nitrogen, carbon, hydrogen and oxygen. Such reflectance is extremely useful when evaluating qc of materials.


Lisun Electronics Inc was found by Lisun Group in 2003. We have sales & service offices in Shanghai, Russia, India and Korea. In 2012, we built a high level products show room & lab center in Shanghai. Lisun Group has set up a new manufactory in China to research and develop the world high-technology CFL & LED Test Instruments. Its quality system has been strictly certified by ISO9001:2008. The Lisun Group products have been authenticated by the third party lab and were awarded by CE certificate. As a CIE Supportive Member, all of the test instruments produced by Lisun Group are designed according to CIE Standards.

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